Coding

Part:BBa_J100399:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2018-05-18)


mScarlet-i


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Avoid internal PstI, BamHI, Bsa I restriction sites.


Source

https://www.ncbi.nlm.nih.gov/protein/APD76536.1

References